Cell line development meets Raman spectroscopy
KBI Biopharma’s monitoring of a diverse array of cell lines and cell concentrations in minimal volume for automated cell cultivation
With Endress+Hauser's support, KBI Biopharma has developed a robust predictive model using a Raman analyzer system to monitor CHO-M cell cultures. This non-invasive technique, requiring smaller sample volumes than traditional methods, is ideal for cell concentration monitoring in cell line development. KBI Biopharma plans to expand the use of Raman technology for broader upstream processing applications and extended process parameter monitoring of CHO cell cultures.
Benefits:
-
Easy-to-use system: Real-time data acquisition and online process provides valuable process insights with no complex modeling and an intuitive display of results
-
Production cost reduction and sustainability: Minimal analysis reagents and a consumable-free design helps to lower production costs. Cell counting is typically performed to monitor cell growth rates throughout the routine cell passaging process
-
Process automation compatibility: The Raman flow assembly allows for a seamless integration into future automated cell cultivation platforms
The expertise and support from the Raman application scientists at Endress+Hauser were instrumental in advancing our innovative cell line development.
KBI Biopharma
Customer challenges
KBI Biopharma focuses on enhancing its process automation at different stages of a research cell bank generation, taking into consideration key criteria:
Low volume: At early cell line development stages, volumes of cultures are within the milliliter range.
High-throughput: Hundreds of isolated clone candidates need to be screened to select the right producer.
Speed: Reducing timelines in cell line development processes is critical to accelerate the biologic’s development path.
Our solution
KBI Biopharma chose to partner with Endress+Hauser to screen for cell growth performances during CLD using Raman spectroscopy, from the design of the experiments down to chemometrics modeling support and training. The selected Raman system is tailored for small volume and flow paths, leveraging signal amplification and low-noise technology to deliver fast results. It consists of the following components:
Raman Rxn2 analyzer
Rxn-10 probe
Raman flow assembly
相关产品组合
| FLEX产品选型 | 仪表技术性能 | 选型便捷程度 |
|---|---|---|
Fundamental选型 完美胜任基本测量任务 |
仪表技术性能
|
选型便捷程度
|
Lean选型 轻松应对关键工艺的测量挑战 |
仪表技术性能
|
选型便捷程度
|
Extended选型 创新技术助力工艺流程优化 |
仪表技术性能
|
选型便捷程度
|
Xpert选型 满足严苛工况的特殊测量要求 |
仪表技术性能
|
选型便捷程度
特殊选型 |
